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Invitrogen™ eBioscience™ Fura-2 AM Dye

Catalog No. 5016958
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5016958 1 mg
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Catalog No. 5016958 Supplier Invitrogen™ Supplier No. 65085839
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Description

Imaging dye that binds to free Ca2+. Fura-2 is the preferred dye for ratiometric imaging microscopy with digital image analysis, especially when altering excitation wavelengths is more practical than detecting multiple emission wavelengths.

  • Upon binding Ca2+, the excitation spectrum of Fura-2 shifts to shorter wavelengths between 300 and 400nm, while the peak emission remains steady around 510nm
  • The Kd of Fura-2 is highly dependent on pH, temperature, ionic strength and viscosity of the cytosol, thus great care should be taken when non-standard conditions are used
  • Molecular weight: 1001.86g/mol
  • Peak excitation: variable depending on the concentration of free Ca2+, between 300 and 400nm
  • Peak emission: 510nm
  • Reconstitute in high-quality, freshly opened DMSO. Once reconstituted, protect from light and store at −20°C. Avoid freeze-thawing

Specifications

Detection Method Fluorescence
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Product Type Dye
Emission 300 to 400/510
Product Line eBioscience
Quantity 1 mg

For Research Use Only. Not for use in diagnostic procedures.