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Gibco™ FreeStyle™ MAX Reagent

Catalog No. 16447500
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15 mL
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Catalog No. 16447500 Supplier Gibco™ Supplier No. 16447500

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Animal origin-free formulation for transfecting plasmid DNA into eukaryotic cells that can easily be scaled up to produce large amounts of recombinant proteins

  • Allows highest expression levels and transfection rates with lowest cytotoxicity in bio-production applications
  • Formulated for use with:
  • FreeStyle 293-F Cells (suspension human embryonal kidney cells, Cat. No. R790-07) in serum-free FreeStyle 293 Expression Medium (Cat. No. 12338-018)
  • FreeStyle CHO-S Cells (suspension Chinese Hamster Ovary cells, Cat. No. R800-07) in serum-free FreeStyle CHO Expression Medium (Cat. No. 12651-014)
  • DG44 Cells (DHFR– suspension CHO cells) in CD DG44 Medium with 8 mM L-glutamine and 18 mL/L 10% Pluronic F-68 (Cat. No. A11000-01)
  • FreeStyle™ MAX Reagent is a proprietary, animal origin–free formulation for transfecting plasmid DNA into eukaryotic cells that can easily be scaled up to produce large amounts of recombinant proteins
  • Intended for use with the FreeStyle™ MAX 293 Expression System (Cat. No. K9000-10) and the FreeStyle™ MAX CHO Expression System (Cat. No. K9000-20).

Specifications

Classification Animal Origin-Free
Content And Storage Each unit contains one vial of reagent. Store at +4°C. FreeStyle™ MAX is guaranteed stable for 6 months when stored properly.
Cell Type Established Cell Lines
Sample Type Plasmid DNA
Transfection Technique Lipid-based Transfection
For Use With (Application) Transfection
High-throughput Compatibility Not High-throughput Compatible (Manual)
Product Line Freestyle
Product Type Transfection Reagent
Quantity 15 mL
Serum Compatible Yes
Shipping Condition Wet Ice
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Frequently Asked Questions (FAQs)

What is the advantage of FreeStyle Max Reagent over 293fectin Transfection Reagent?

Both FreeStyle Max Reagent and 293fectin Transfection Reagent provide similar levels of high transfection efficiency; however, FreeStyle Max Reagent has lower cytotoxicity and hence results in higher protein yields. Additionally, FreeStyle Max Reagent is animal-origin free.

Can FreeStyle 293-F cells grown in FreeStyle 293 expression medium be transfected with ExpiFectamine293 Reagent along with enhancers? Is so, will the transfection efficiency be higher than that with FreeStyle Max Reagent?

You should be able to use the ExpiFectamine293 Reagent for transfection of Freestyle 293-F cells grown in FreeStyle 293 Expression Medium; however, the enhancers will provide only a little boost in expression as they are designed to work with higher density cultures that FreeStyle 293 Expression Medium cannot support.

Can FreeStyle MAX reagent be used for transfecting adherent cells?

Yes, the reagent can be used for adherent cells, however the protocol supplied with the reagent has been optimized for CHO and 293 cells in suspension culture. If you want to transfect adherent cells, please use Lipofectamine 2000 transfection reagent.

For Research Use Only. Not for use in diagnostic procedures.