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Gibco™ FreeStyle™ F17 Expression Medium DFS Item

An animal origin-free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation.

$255.00 - $3860.00

Specifications

Culture Environment CO2
Cell Line HEK293
Classification Animal Origin-free, Chemically-defined, Protein-free, Serum-free
Concentration 1 X
Culture Type Suspension Cell Culture
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Products 3
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
50-591-354 DFS Item
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Gibco™
A1383501
1 L
Each for $255.00
 
A1383504 DFS Item
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Gibco™
A1383504
20 L
Each for $3,860.00
 
A1383503 DFS Item
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Gibco™
A1383503
10 L
Each for $2,085.00
 
Description

Description

FreeStyle™ F17 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation. This is a multi-platform medium compatible with a range of transient expression systems. The addition of 4–8 mM L-glutamine or GlutaMAX™ supplement is required before use. Gibco™ FreeStyle™ F17 Expression Medium features:

  • Little or no required adaptation
  • Ability to support suspension growth and transfection of 293-F and CHO cells
  • Animal origin–free, protein-free, and chemically defined formulation
  • Scalability in spinner flasks and bioreactors

    Little or no required adaptation
    Gibco™ 293-F, 293-H, FreeStyle™ 293-F, and CHO-S cells in suspension can be cultured directly into Gibco™ FreeStyle™ F17 Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco™ FreeStyle™ F17 Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations.

    Ability to support suspension growth and transfection of 293-F and CHO cells
    Gibco™ FreeStyle™ F17 Medium provides equal or better performance compared to Gibco™ FreeStyle™ 293 Expression Medium and Gibco™ FreeStyle™ CHO Medium. Gibco™ FreeStyle™ F17 Medium supports 293-F cells at a peak density of 6–7 × 106 viable cells per mL, and supports CHO-S cells at a peak density of 8–9 × 106 viable cells per mL. Cells cultured in Gibco™ FreeStyle™ F17 can be transfected using a wide variety of transfection reagents, including lipid-based transfection reagents, such as 293fectin™ and Freestyle™ MAX Reagent. Protein expression typically exceeds 75–150μg/mL IgG in 293-F cells.

    Animal origin–free, protein-free, and chemically defined formulation
    Gibco™ FreeStyle™ F17 Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco™ chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

    Scalability in spinner flasks and bioreactors
    Protein production using Gibco™ FreeStyle™ F17 Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco™ FreeStyle™ F17 Medium with additional Pluronic™ F-68 to avoid sheer stress in the culture.

    cGMP manufacturing and quality system
    Gibco™ FreeStyle™ F17 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
  • Specifications

    Specifications

    CO2
    Animal Origin-free, Chemically-defined, Protein-free, Serum-free
    Suspension Cell Culture
    Expression Medium
    Sterile
    Protein expression, viral vector production
    Qualified for Further Manufacturing
    HEK293
    1 X
    Liquid
    Serum-free
    No Glutamine
    Freestyle™
    SDS
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    For Research use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.