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Anti-Mouse/Rat Foxp3 Staining Set FITC, eBioscience™

Catalog No. 5016971
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5016971 1 kit
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Catalog No. 5016971 Supplier Thermo Fisher Scientific Supplier No. 71577540
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Description

Includes

  • Fixation/Permeabilization Concentrate: 30mL (4X) Store at 2-8°C. Avoid agitation. Dilute 1 part Fixation/Permeabilization Concentrate with 3 parts Fixation/Permeabilization Diluent to make the Fixation/Permeabilization working solution. Caution: This solution contains Paraformaldehyde, which is toxic and a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided. Wear proper protective clothing and gloves.
  • Fixation/Permeabilization Diluent: 100mL. Store at 2-8°C. The diluent is intended to be used in combination with the Fixation/Permeabilization Concentrate.
  • Permeabilization Buffer (10X): 100mL. Store at 2-8°C. Dilute to 1X with deionized/distilled water and store at 4°C. Caution: Harmful if swallowed or irritant by contact. Wear proper protective clothing and gloves.
  • Anti-Mouse/Rat Foxp3 (FJK-16s): 25μg. Store at 2-8°C.

Antibody Staining Kit

The FJK-16s antibody reacts with mouse, rat, dog, porcine, and bovine Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.

Intracellular staining of mouse splenocytes with FJK-16s using the PE anti-mouse/rat Foxp3 Staining Set and protocol reveals approximately 2% of total cells in the C57Bl/6 strain and approximately 3-5% in the BALB/c mouse strain. Multicolor flow cytometric analysis demonstrates approximately 90% of the CD4+CD25+ cells and 4% of the CD4+CD25- cells staining with FJK-16s. B220+, CD11b+, CD11c+, and Ly6G/Gr-1+ cells do not show significant co-staining with FJK-16s.

Please see our FAQ regarding the usage of eBioscience Foxp3 reagents.

Not included:
Fc Block (cat. 14-0161)
Flow Cytometry Staining Buffer (cat. 00-4222)
Rat IgG2a isotype Control (cat. 12-4321, 11-4321, or 17-4321)

Specifications

Antigen Foxp3
Applications Flow Cytometry
Classification Monoclonal
Clone FJK-16s
Concentration 0.5mg/mL
Conjugate FITC
Host Species Rat
Quantity 1 kit
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse, Rat
Content And Storage Store at 2-8°C. Light sensitive material. Use within 6 months of opening or by date indicated on the bottle.
Isotype IgG2a κ
Product Line eBioscience
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For Research Use Only. Not for use in diagnostic procedures.