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Anti-Human Foxp3 Staining Set Alexa Fluor™ 488 , eBioscience™

Catalog No. 5016978
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5016978 1 kit
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Catalog No. 5016978 Supplier Thermo Fisher Scientific Supplier No. 73577640
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Description

Includes

  • Fixation/Permeabilization Concentrate (4X) (cat. 00-5123): 30mL, store at 2-8°C. Avoid agitation.
  • Fixation/Permeabilization Diluent (cat. 00-5223): 100mL, store at 2-8°C.
  • Permeabilization Buffer (10X) (cat. 00-8333): 100mL, store at 2-8°C. Note: The 10X Permeabilization Buffer has a natural tendency to precipitate, however, its function is not affected by this. To clarify, the solution can be filtered after dilution to 1x working solution.
  • Rat IgG2a K Isotype Control Alexa Fluor 488 (cat. 73-4321): 25 tests, store at 2-8°C. Light-sensitive material.
  • Anti-Human Foxp3 Alexa Fluor 488 (clone PCH101, cat. 53-4776): 25 tests, store at 2-8°C. Light-sensitive material.

Antibody Staining Kit

This Anti-Human Foxp3 Staining Set contains the buffers and monoclonal antibody necessary to successfully stain and identify Foxp3+ cells. The PCH101 monoclonal antibody reacts with the amino terminus of human Foxp3, also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3 is a 49-55kDa protein and a member of the forkhead/winged-helix family of transcription factors. It was identified as the gene responsible for the X-linked lymphoproliferative disease observed in scurfy (sf) mice and in the human disorder, X-linked autoimmunity-allergic dysregulation syndrome (XLAAD). Constitutive expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

The PCH101 antibody crossreacts with rhesus, chimpanzee, and cynomolgus Foxp3. PCH101 recognizes a different epitope of Foxp3 than clones 236A/E7 and 150D/E4.

Specifications

Antigen Foxp3
Applications Flow Cytometry
Classification Monoclonal
Clone PCH101
Conjugate Alexa Fluor 488
Gene Alias Forkhead Box P3, Scurfin, JM2
Host Species Rat
Quantity 1 kit
Regulatory Status RUO
Primary or Secondary Primary
Target Species Chimpanzee, Cynomolgus, Human, Macaque, Monkey, Non-human Primate, Rhesus Monkey
Content And Storage Store at 2-8°C. Do not freeze. Light sensitive material. Use within 6 months of opening or by date indicated on the bottle.
Isotype IgG2a κ
Product Line eBioscience
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For Research Use Only. Not for use in diagnostic procedures.