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  6. Invitrogen™ FOXP3 Monoclonal Antibody (FJK-16s), Brilliant Violet™ 711, eBioscience™

Invitrogen™ FOXP3 Monoclonal Antibody (FJK-16s), Brilliant Violet™ 711, eBioscience™

Catalog No. 407577380
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25 μg
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407577380 25 μg
407577382 100 μg
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Catalog No. 407577380 Supplier Invitrogen™ Supplier No. 407577380
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Description

Rat Monoclonal Antibody

Description The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human. Please note that FJK-16s has been optimized for use with the Foxp3/Transcription Factor Buffer Staining Set (Product # 00-5523-00). The use of other fixation and staining buffers is not recommended. Applications Tested This FJK-16s antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) a...

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

Specifications

Antigen FOXP3
Applications Flow Cytometry
Classification Monoclonal
Clone FJK-16s
Concentration 0.2 mg/mL
Conjugate Brilliant Violet 711
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Foxp3
Gene Accession No. Q99JB6
Gene Alias AIID; DIETER; forkhead box P3; forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; forkhead/winged helix transcription factor 3; Foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; regulatory protein Foxp3; RGD1562112; RP23-54C14.1; scurfin; scurfy; sf; XPID
Gene Symbols Foxp3
Host Species Rat
Purification Method Affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 100037405, 20371, 317382, 444998, 491876, 506053
Target Species Bovine, Canine, Feline, Mouse, Pig, Rat
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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