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Invitrogen™ Folic Acid Competitive ELISA Kit

Catalog No. EEL149
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96 Tests
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Catalog No. Quantity
EEL149 96 Tests
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Catalog No. EEL149 Supplier Invitrogen™ Supplier No. EEL149

Description

ELISA

The Folic Acid/Vitamin B9 (FA/VB9) ELISA quantitates FA/VB9 in serum, plasma and other biological fluids. Principle of the method The Competitive multispecies ELISA research use only kit is designed to quantitatively measure Folic Acid/Vitamin B9 (FA/VB9) independent of species. A FA/VB9 standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to FA/VB9 is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of FA/VB9 in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Folic acid, a B-vitamin essential for numerous biochemical processes, plays a key role in DNA synthesis and repair, as well as methylation, which is crucial for genome regulation and normal mammalian development. It functions primarily as a methyl-group donor and is involved in critical biological processes such as cell division and gene expression regulation. This vitamin is integral to folate metabolism, which has significant implications for DNA methylation and thereby impacts epigenetic modifications. Variations in dietary intake and functional polymorphisms in the genes encoding folate metabolism enzymes can influence its efficacy, making it a potential factor in cancer susceptibility and other diseases. Folic acid deficiency is common and can arise from poor diet, malabsorption, alcohol use, obesity, and kidney failure. Due to its protective effects against neural tube defects and ischemic events, folic acid fortification in foods has been adopted in many countries. The molecular interaction of folic acid with DNA and RNA occurs at different sites, highlighting its multifaceted role in cellular processes. Overall, folic acid's impact on DNA methylation underscores its importance in health and disease prevention.

Specifications

Assay Range 1.56-100 ng/mL
Assay Sensitivity 0.94 ng/mL
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Cell Lysates, Plasma, Serum, Tissue Homogenates
Target Species All Species
For Use With (Equipment) Absorbance Microplate Reader
Interassay CV <10%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Label or Dye Biotin
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Tissue Homogenate 50 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Refer to product documentation for component specific storage temperature.
Target Folic Acid
Test Time 1 hr 20 min
Total Assay Time 2 hr 30 min
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