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Gibco™ FoamAway™ Irradiated AOF (animal origin-free) Antifoaming Agent

Catalog No. A1036902
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0.5 L in a 1 L bag
2.5 L in a 5 L Bag
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Catalog No. Quantity
A1036902 0.5 L in a 1 L bag
A1036901 2.5 L in a 5 L Bag
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Catalog No. A1036902 Supplier Gibco™ Supplier No. A1036902
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Description

FoamAway™ Irradiated AOF (animal origin-free) Antifoaming Agent

FoamAway™ Irradiated AOF is a ready-to-use antifoaming agent designed to offer high performance and convenience in combating foam in your cell culture system. It is prediluted, presterilized, and packaged to connect directly to your bioreactor with no further processing required.

Specifications

Classification Animal Origin-Free
Content And Storage Store at room temperature. Shake well before use. Do not filter.
Culture Type Suspension Cell Culture
For Use With (Application) Cell Culture
Product Line Gibco
Product Type Antifoaming Agent
Phenol Red Indicator No Phenol Red
Quantity 0.5 L in a 1 L bag
Shipping Condition Room Temperature
Volume (Metric) 500 ml
Form Liquid
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Frequently Asked Questions (FAQs)

What is the tolerance for the target simethicone concentration in FoamAway Irradiated AOF (animal origin-free) Antifoaming Agent and what is the maximum concentration it can have?

The target shipping percentage of simethicone in FoamAway Irradiated AOF (animal origin-free) Antifoaming Agent is 3%. This supports the defoaming assay noted on the COA for the shelf life of the product.

FoamAway Irradiated AOF (animal origin-free) Antifoaming Agent is formulated to a 4.0% simethicone concentration during manufacture, representing the upper end of the product's concentration range, to account for loss of simethicone during processing.
The final concentration of simethicone may be variable in the finished product, but is ≤4.0%. The final product must pass a defoaming activity assay as a quality release criterion, ensuring defoaming activity throughout the 8-month shelf life.

Why does the FoamAway Irradiated AOF Antifoaming Agent (Cat. No. A1036901, A1036902) have a shelf life of 12 months? Do you have any data on extending the expiry time frame?

Thermo Fisher Scientific conducted extended stability testing showing that FoamAway Irradiated AOF Antifoaming Agent remains within specification for 12 months from the date of irradiation. At the 13-month time point, multiple lots met the defoaming activity specification (≤ 15 seconds). Thus, 12 months is the supported shelf life based on validated stability data./
What type of connectors are used in FoamAway Irradiated AOF (animal origin-free) Antifoaming Agent?

The connectors are male and female luer locks.

What is your usage recommendation for FoamAway Irradiated AOF (animal origin-free) Antifoaming Agent?

We recommend shaking to thoroughly mix the product before each addition. Approximately 1:1,000 dilution may be the midrange of the working concentration, about 30 ppm. The range may be about a 1:20,000 to 1:600 depending on the process. For an extended culture, we recommend re-adding material during the run as it is incorporated with the foaming components; we recommend adding as little as possible to keep the downstream processes functioning.

For Research Use or Further Manufacturing. Not for use in diagnostic procedures.