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Invitrogen™ Fluorescein/Oregon Green Polyclonal Antibody
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Catalog No. A889
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500 μL
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A889 500 μL
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Catalog No. A889 Supplier Invitrogen™ Supplier No. A889

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Description

Rabbit Polyclonal Antibody

Fluorescein/Oregon Green Polyclonal Antibody for Flow, ICC/IF, IHC

Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes.

Specifications

Antigen Fluorescein/Oregon Green
Applications Flow Cytometry, Immunohistochemistry, Immunocytochemistry
Classification Polyclonal
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation 0.1M potassium phosphate with 5mM sodium azide; pH 8
Gene Alias FITC
Host Species Rabbit
Immunogen Fluorescein/Oregon Green.
Purification Method Purified
Quantity 500 μL
Regulatory Status RUO
Primary or Secondary Primary
Target Species Chemical
Content And Storage 4°C
Product Type Antibody
Form Liquid
Isotype IgG
Target Molecule Chemical
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Frequently Asked Questions (FAQs)

I want to label F-actin with phalloidin and image with transmission electron microscopy. What is the best way to go about it?

One way is to use a biotin-phalloidin conjugate, then label that with a colloidal gold conjugate of streptavidin. You will need to block endogenous biotin first. Another method is to label with fluorescein phalloidin, then with an anti-fluorescein antibody conjugated with colloidal gold.