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Molecular Probes™ Fluo-4, Pentapotassium Salt, cell impermeant

Catalog No. F14200
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500 μg
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F14200 500 μg
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Catalog No. F14200 Supplier Molecular Probes™ Supplier No. F14200
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Description

Fluo-4, Pentapotassium Salt, cell impermeant

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. Fluo-3 has been used to image the spatial dynamics of Ca2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipette, microinjection, or our Influx™ pinocytotic cell-loading reagent. These indicators are useful for fluorescence and confocal microscopy, flow cytometry, and microplate screening applications.

Calcium Indicator (Cell-Impermeant Salts) Specifications:

  • Label (Ex/Em of Ca2+–bound form): Fluo-4 (494/516 nm)

  • Fluorescence intensity increase upon binding Ca2+: >100 fold

  • Kd for Ca2+ in buffer: ∼335 nM

  • Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength

    Using TPEN to Control Heavy Metal Cations

    In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+. Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN.

    More Choices for Fluorescent Calcium Indicators

    We offer a large selection of Molecular Probes™ calcium indicators for use in various experimental scenarios. For more information, review Fluorescent Ca2+ Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes™ Handbook.

    For UV-excitable Ca2+ indicators, protein-based Ca2+ indicators, conjugates of Ca2+ indicators, and for fluorescence-based indicators of other metal ions (i.e., Mg2+, Zn2+) review Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions—Chapter 19 in the Molecular Probes™ Handbook.

    For Research Use Only. Not for human or animal therapeutic or diagnostic use.

    • Specifications

    Content And Storage Store in freezer -5°C to -30°C and protect from light.
    Detection Method Fluorescence
    For Use With (Equipment) Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
    Product Type Calcium Indicator
    Dye Type Fluorescent Dye-Based
    Quantity 500 μg
    Shipping Condition Room Temperature

    For Research Use Only. Not for use in diagnostic procedures.