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Gibco™ Floor Plate Cell Expansion Kit

Catalog No. A3165801
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Description

The Floor Plate Cell Expansion Kit is a component of the Gibco™ PSC Dopaminergic Neuron Differentiation Kit that is also offered separately.

It enables additional expansion of floor plate progenitor cells generated using complete Floor Plate Cell Specification Medium (part of the Differentiation kit).

Note: Each additional expansion beyond passage 2 will result in an additional 4–6-fold increase in the total number of floor plate cells without compromising the quality of TH neurons (positive for both TH and FOXA2). Further expansion (up to passage 10) results in stable expansion of progenitor cells, but the resulting TH neurons may show decreased FOXA2 expression.

Specifications

Quantity 1 kit
Content And Storage • 1 x 500 mL Floor Plate Cell Expansion Base
• 1 x 10 mL Floor Plate Cell Expansion Supplement
Cell Type Stem Cells (Neural)
Shipping Condition Dry ice and gel pack

Frequently Asked Questions (FAQs)

Is cell density in Floor Plate Specification medium critical for Dopaminergic neuron differentiation?

Yes, cell density should be optimized to attain full confluency at day 7 in the specification medium to have an efficient differentiation.

Can Dopaminergic Neuron Maturation medium be used to differentiate neural stem cells?

We have tested neural stem cells (NSCs) isolated from fetal tissue or derived from pluripotent stem cells (PSCs), and have seen that both populations can benefit from maturation medium (DMEM/F12 + Dopaminergic Neuron Maturation Supplement) to have nicely spread homogenous neurons with reduced progenitor population. Matured neurons can be further maintained in neurobasal medium supplemented with Dopaminergic Neuron Maturation Supplement.

Do you offer Dopaminergic Neuron differentiation service?

Yes. Please go here (https://www.thermofisher.com/us/en/home/life-science/stem-cell-research/stem-cell-services/stem-cell-differentiation-services.html).

For Research Use Only. Not for use in diagnostic procedures.