missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PV4610

Thermo Scientific™ FL - eA2 Peptide, 100μM

Catalog No. PV4610
Encompass
Change view
Click to view available options
Quantity:
100 μL
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PV4610 100 μL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. PV4610 Supplier Thermo Scientific™ Supplier No. PV4610
Only null left

Description

Contain known interaction motifs and are labeled with fluorescein

  • LanthaScreen™ fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein.
  • These peptides are matched and validated to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays.
  • Assays developed using these reagents enable primary or secondary screening of nuclear receptor agonists and/or antagonists.
  • Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events, including dissociation of repressor proteins, association of coactivator proteins, and assembly of pol II and other transcriptional factors for activation of target genes.
  • TR-FRET based assays can be developed using the LanthaScreen™ panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding, either by determining the affinity of ligand-bound receptor for different coregulator peptides, or by identifying additional agonists or antagonists via displacement or recruitment of a specific coregulator peptide.
  • In the LanthaScreen™ TR-FRET RXR beta Coactivator Assay, a terbium (Tb)-labeled anti-GST antibody is used to indirectly label GSTtagged RXR beta protein.
  • An agonist (9-cis Retinoic Acid) added to the receptor which, upon ligand binding, causes a conformational change resulting in recruitment of the Fl-D22 coactivator peptide (Figure 1). The binding of the fluorescent coregulator peptide to RXR beta causes an increase in the TR-FRET emission ratio (Figure 2).
  • LanthaScreen™ fluorescent nuclear receptor coregulator peptides are supplied as 100 μM solutions in peptide-appropriate buffers. Store peptides at -20°C.

Specifications

Quantity 100 μL
Product Type Peptide
Content And Storage Store in freezer (-5 to -30°C).
Shipping Condition Dry Ice
Label Type Classic Dyes

For Research Use Only. Not for use in diagnostic procedures.