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  6. Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 660

Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 660

Catalog No. 5016964
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5016964 500 tests
501128848 100 tests
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Catalog No. 5016964 Supplier Invitrogen™ Supplier No. 65086418
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Description

Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.

  • Fixable Viability Dyes may be used to label cells from all species
  • Fixable Viability Dye eFluor™ 660 can be excited by the red (633nm) laser line and has a peak emission of 660nm that can be detected using a 660/20 band pass filter (equivalent to APC or Alexa Fluor™ 647)
  • For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye
  • If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute. After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye.
  • Fixable Viability Dye eFluor™ 660 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO. It should be protected from light and moisture
  • Store at −70°C with dessicant
  • It may be freeze-thawed up to 20 times
  • Allow vial to equilibrate to room temperature before opening
  • Staining may be done before or after surface staining
  • Cells may be cryopreserved after staining with no adverse effect on staining intensity of dead cells after thawing

Specifications

Color Red
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Flow Cytometer
Product Type Dye
Emission 633/660
Product Line eBioscience
Quantity 500 tests

For Research Use Only. Not for use in diagnostic procedures.