Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 450

Description

• Unlike 7-AAD and propidium iodide, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized, and stained for intracellular antigens without any loss of staining intensity of the dead cells
• Thus, using these dyes allows dead cells to be excluded from analysis when intracellular targets are being studied
• Fixable Viability Dye eFluor™ 450 can be excited by the violet (405 nm) laser line and has a peak excitation of 450 nm that can be detected using a 450/50 band pass filter (equivalent to eFluor™ 450 or Pacific Blue™)
• For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye
• If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute
• After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye
• Fixable Viability Dye eFluor™ 450 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO
• It should be protected from light and moisture
• Store at -70°C with dessicant, it may be freeze-thawed up to 20 times
• Allow vial to equilibrate to room temperature before opening