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PeproTech FGF10 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Description
AA Sequence of recombinant protein: MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human FGF-10. Anti-Human FGF-10-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human FGF-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human FGF-10 (500-P151G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human FGF-10. Western Blot: To detect hFGF-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hFGF-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P151GBT-1MG will be provided as 2 x 500 μg
Specifications
Specifications
| Antigen | FGF10 |
| Applications | ELISA, Western Blot |
| Classification | Polyclonal |
| Concentration | 0.1-1.0 mg/mL |
| Conjugate | Biotin |
| Formulation | PBS with no preservative |
| Gene | FGF10 |
| Gene Accession No. | O15520 |
| Gene Alias | AEY17; BB213776; FGF; Fgf10; FGF-10; Fibroblast growth factor; Fibroblast growth factor 10; Gsfaey17; Keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria |
| Gene Symbols | FGF10 |
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