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Invitrogen™ Human FGF1 (FGF alpha) ELISA Kit
ELISA
$888.00
Specifications
| Accession Number | P05230 |
|---|---|
| Assay Range | 16.38 to 4000 pg/mL |
| Assay Sensitivity | 12 pg/mL |
| Calibration Range | 12 pg/mL to 4000 pg/mL |
| Assay | Solid-phase sandwich ELISA |
Description
The Human Fibroblast Growth Factor 1 (Hu FGF1/FGFα) ELISA quantitates Hu FGF1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FGF1. Principle of the method The Human FGF1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
FGF1 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.Specifications
| P05230 | |
| 12 pg/mL | |
| Solid-phase sandwich ELISA | |
| ELISA | |
| Plasma, Serum, Supernatant | |
| Human | |
| Colorimetric Microplate Reader | |
| AFGF,ECGF,ECGF-beta,ECGFA,ECGFB,FGF-1,FGF-alpha,FGFA,GLIO703,HBGF-1,HBGF1 | |
| <10 | |
| HRP | |
| Angiogenesis | |
| 2°C to 8°C | |
| FGF1 | |
| 4 hrs 45 mins |
| 16.38 to 4000 pg/mL | |
| 12 pg/mL to 4000 pg/mL | |
| HRP | |
| Growth Factors & Receptors | |
| This ELISA kit shows no cross-reactivity with the following cytokines tested: human BLC, BDNF, bFGF, BMP-4, BMP-5, BMP-7, b-NGF, Eotaxin-1, Eotaxin-2, FGF-4, FGF-7, G-CSF, GM-CSF, I-309, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 ra, IL-2, IL-4, IL-5, IL-6, IL-6 sR, IL-7, IL-8, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-16, IL-17, MCP-1, M-CSF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PDGF-BB, RANTES, sTNFRI, TIMP-1, TIMP-2, TNF alpha, TNF beta, VEGF, VEGFR2, VEGFR3. | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 2246 | |
| <12% | |
| Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| RUO | |
| Plasma 50 μL, Serum 50 μL, Supernatant 100 μL | |
| AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-1, FGF-alpha, FGFA, GLIO703, HBGF-1, HBGF1 | |
| 1 hrs 20 mins |