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Invitrogen™ Human FGF-7 (KGF) ELISA Kit
Description
The Human Fibroblast Growth Factor 7/KGF (Hu FGF7) ELISA quantitates Hu FGF7 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FGF7. Principle of the method The Human FGF7 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Specifications
Specifications
| Accession Number | P21781 |
| Assay Range | 0.55 to 400 pg/mL |
| Assay Sensitivity | 0.5 pg/mL |
| Calibration Range | 0.5 pg/mL to 400 pg/mL |
| Assay | Solid-phase sandwich ELISA |
| Conjugate | HRP |
| Product Type | ELISA |
| Protein Family | Growth Factors & Receptors |
| Sample Type | Plasma, Serum, Supernatant |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
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