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Gibco™ Fetal Bovine Serum, MaxSpec

Catalog No. A4766801
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A4766801 500 mL
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Catalog No. A4766801 Supplier Gibco™ Supplier No. A4766801
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Description

Gibco MaxSpec Fetal Bovine Serum (FBS) is our highest quality FBS, meeting selective specifications to help provide consistent, reproducible results.

Gibco MaxSpec Fetal Bovine Serum (FBS) is our highest quality FBS, meeting selective specifications to help provide consistent, reproducible results. Only MaxSpec FBS delivers our best quality specifications and testing levels.

Gibco sera: trusted quality for consistent performance

MaxSpec Sera Features

  • Use with a broad range of cell types, especially sensitive cell lines
  • Our most characterized sera with the lowest endotoxin and hemoglobin levels
  • Endotoxin level: ≤1 EU/mL
  • Hemoglobin level: ≤15 mg/dL
  • Origin: United States, Australia, or New Zealand (refer to CoA for specific origin)

Additional features of this serum include:

  • Meets USP/EP guidelines with up to 96 harmonized quality specification tests
  • Additional proliferation tests on top six cell lines
  • EMA virus testing upon request
  • Lowest risk of BSE and lower viral risk
  • ISO 13485-certified; processed in FDA-registered facilities
  • Triple-filtered at 0.1 μm
  • Gibco bottle is easy to use in a hood, helps reduce risk of contamination, and allows you to perform cell culture more consistently
  • Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook

Specifications

Certifications/Compliance Meets USP/EP guidelines; ISO 13485 Certified
Classification Specialty
Content And Storage Store at or below -10°C.
Species Cattle/Bovine
Cell Type Use for a broad range of cell types, especially sensitive cell lines
Age Fetal
Country of Origin Refer to CoA for specific origin
Form Liquid
Format Bottle
Product Type Fetal Bovine Serum
Quantity 500 mL
Sterility Sterile
Sterilization Method Triple-filtered, 0.1 μm
Tested For Meets USP/EP guidelines with up to 96 harmonized quality specification tests; Additional proliferation tests on top 6 cell lines; EMA virus testing upon request
Endotoxin Concentration ≤1 EU/mL
Green Features Less waste
Hemoglobin Concentration ≤15 mg/dL
Purity or Quality Grade Premium Plus
Shipping Condition Frozen
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Frequently Asked Questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.