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  6. Gibco™ Fetal Bovine Serum, exosome-depleted

Gibco™ Fetal Bovine Serum, exosome-depleted
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Catalog No. A2720803
Encompass
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Quantity:
50 mL
500 mL
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Bottle
One Shot
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Catalog No. Quantity Format
A2720803 50 mL One Shot
A2720801 500 mL Bottle
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Catalog No. A2720803 Supplier Gibco™ Supplier No. A2720803
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Description

Gibco™ Exosome-Depleted FBS streamLines and standardizes cell culture workflows for exosome research. It is ready to use and performance tested to help save time and enable optimal exosome preparations.

Gibco™ Exosome-Depleted FBS streamLines and standardizes cell culture workflows for exosome research. It is ready to use and performance tested to help save time and enable optimal exosome preparations.

  • Lowest exosome background
  • Enables consistent results: each lot tested for cell culture performance
  • No preparation needed: ultracentrifugation is not required

Exosome-Depleted FBS has been depleted of ≥90% of endogenous exosomes and is intended for use in cell culture exosome isolation assays. Every lot is tested for exosome depletion and culture performance, and our standard quality control assays are conducted to help ensure optimal and consistent results. This ready-to-use FBS for cell culture exosome isolations helps eliminate the risks associated with FBS reagent preparation by ultracentrifugation, for optimal assay results and increased experimental throughput.

Specifications

Content And Storage Storage conditions: ≤-10°C
Shipping conditions: Frozen
Species Bovine
Age Fetal
Country of Origin United States
Form Liquid
Format One Shot
Product Type Fetal Bovine Serum
Quantity 50 mL
Serum Treatment Exosome-depleted
Sterility Sterile
Product Line One Shot
Shipping Condition Frozen
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Frequently Asked Questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.
Have you tested Exosome-Depleted FBS for mycoplasmas?

Yes, the material undergoes USP sterility testing, which includes mycoplasma testing.
What is the source material for Exosome-Depleted FBS?

Exosome-Depleted FBS is made from Gibco FBS (qualified, US-origin) in the Value category.
How long can serum be stored at 2-8 degrees C?

Serum can be kept at 2-8 degrees C for up to 4 weeks.

Are there any other packaging configurations available for the new One Shot FBS product?

Yes, we also offer a 10 X 50 mL package, which is equivalent to a 500 mL bottle of FBS.

How is the new One Shot FBS bottle different from our existing One Shot bottle?

Our previous One Shot bottle, a 60 mL Nalgene media bottle, is not designed for easy pipetting and pouring whereas the new One Shot FBS bottle has a propietary design that was developed specifically for FBS use. This design allows you to freeze, thaw and pour without the need to aliquot, which minimizes the amount of FBS handling and reduces the risk of contamination.

Does the new One Shot FBS bottle fit into a conical tube rack?

The new One Shot FBS bottle is not shaped to fit into a traditional conical tube rack. It does however fit nicely in a 30 mm test tube rack for the purposes of storage and use in a water bath and biological safety cabinet.
What is the best way to hold the new One Shot FBS bottle for pouring?

For optimal, burp-free pouring, securely grip the bottle using the indents and pour with the chamfered edge facing down.
What is the shelf life of the new One Shot FBS?

The initial shelf life/stability for the new One Shot FBS is two years from the date of manufacture. Additional updates will continue to be made as data becomes available.
Can I heat inactivate the serum in the new One Shot FBS bottle?

Yes, the serum in the new One Shot FBS bottle can be heat inactivated at 56 degrees C for 30 minutes.

Can I reuse the new One Shot FBS bottles?

It is at the discretion of the customer to reuse these bottles for purposes other than what they are intended for. But please note that they cannot be autoclaved.
What type of plastic is the new One Shot FBS bottle made from?

It is made from polyethylene terephthalate (PET, PETE). This is the same plastic used in our other bottles.

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.