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Invitrogen™ Ferritin Human ELISA Kit DFS Item

Sandwich ELISA Kit

Supplier:  Invitrogen™ EHFTL

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Catalog No. EHFTL



Description

Description

The Human Ferritin ELISA quantitates Hu Ferritin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Ferritin. Principle of the method The Human Ferritin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. Ferritin is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in the light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. The ferritin gene has multiple pseudogenes, and ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis. Ferritin is a holoenzyme shell with a molecular weight of about 450 kDa, and consists of 24 subunits of two types, H(heavy) and L(light), and capable of storing up to 4,500 atoms of ferric iron.
Specifications

Specifications

P02792,P02794
600 pg/mL
Ferritin ELISA Kit, 96 Assays, 1 Kit, Human Species, Other Proteins Protein Family, Inflammation Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Ferritin in Samples Using a Microplate Reader
Solid-phase sandwich ELISA
Colorimetric
Other Proteins
This Ferritin ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
96 Assays
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
2495, 2512
Human
<10%
HRP
RUO
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
LFTD, NBIA3
1 hr. 20 min.
0.81-200 ng/mL
0.6ng/mL to 200ng/mL
Ferritin
Biotin
ELISA Kit
Plasma, Serum, Supernatant
TMB
Human
Colorimetric Microplate Reader
FHC,FTH,FTHL6,HFE5,PIG15,PLIF,LFTD,NBIA3
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-Human ferritin precoated 96-well strip plate, lyophilized recombinant human ferritin standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent (5X conc
96 Tests
Inflammation
2°C to 8°C
Human
4 hr. 45 min.
SDS
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