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Thermo Scientific™ TranscriptAid T7 High Yield Transcription Kit

Catalog No. ferk0441
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FERK0441 50 reactions
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Catalog No. FERK0441 Supplier Thermo Scientific™ Supplier No. K0441
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Description

Get high yield in vitro transcription from DNA templates containing a T7 RNA Polymerase promoter, including linearized plasmids or PCR products.

TranscriptAid T7 High Yield Transcription Kit contains reagents for 50 reactions of 20 μL. Each reaction yields up to 200 μg RNA from 1 μg of template in 2 hours (see Figures 1 and 2). The reaction can be scaled up to produce milligram amounts of full-length RNA. The kit can be used to produce both long and short transcripts for applications that require large yields of RNA (see Figure 3). All necessary reagents for transcription are included, as well as the RiboRuler High Range RNA Ladder for sizing and quantification.

Highlights include:

  • Exceptionally high yields—up to 200 μg in 2 hours
  • Versatile—suitable for both short and long RNA transcripts
  • Milligram amounts of RNA in a single, scaled-up reaction
  • Flexible—generates unlabeled, labeled or capped RNA
  • RiboRuler RNA Ladder supplied with kit for sizing and quantification

Specifications

Product Line TranscriptAid
Product Type TranscriptAid T7 High Yield Transcription Kit
Quantity 50 reactions

Frequently Asked Questions (FAQs)

What is the advantage of using TranscriptAid T7 High Yield Transcription Kit over Thermo Scientific T7 RNA Polymerase with a standard transcription buffer?

TranscriptAid T7 High Yield Transcription Kit is designed to produce both long and short transcripts for applications that require large yields of RNA (milligram amounts). The kit can achieve 10 times greater amount than from conventional in vitro transcription reactions with T7 RNA polymerase due to its unique proprietary enzyme and buffer formulations. Please note that TranscriptAid T7 High Yield Transcription Kit is not recommended for generation of radioactively labelled RNA. Due to large quantities of RNA synthesized with the kit, generation of high specific activity radiolabeled probes would require prohibitively large amounts of radiolabeled nucleotide.

For Research Use Only. Not for use in diagnostic procedures.