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  5. Thermo Scientific™ T7 RNA Polymerase (20 U/μL)
  6. FEREP0111

Thermo Scientific™ T7 RNA Polymerase (20 U/μL)

Thermo Scientific™ T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.

Supplier:  Thermo Scientific™ EP0111

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Catalog No. FEREP0111


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Alert:

This chemical may require us to obtain additional information for our regulatory and chemical compliance records. If required, we will contact you for this information once your order is placed.


Description

Description

Thermo Scientific™ Bacteriophage T7 RNA polymerase is DNA-dependent RNA polymerase with strict specificity its respective double-stranded promoter.

It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.
  • Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Recommended for:

Synthesis of unlabeled and labeled RNA that can be used:for hybridization, in vitro RNA translation; as aRNA, siRNA, substrate in RNase protection assays, template for genomic DNA sequencing.

Note:

Consensus promoter sequences: TAATACGACTCACTATA G GGAGA. The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively.

Greener Choice Claims
  • Environmental benefits include:
    • Free-of a substance that causes environmental harm
    • Non-Toxic
    • Ozone-Safe or Safe for the Ozone Layer
    • Recyclable
    • Recycled Content
    • Renewable Energy
    • Renewable Materials
Specifications

Specifications

T7 RNA Polymerase
5,000 units
20U/µL
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For Research Use Only. Not for use in diagnostic procedures.