Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.
- Efficient synthesis of full-length first strand cDNA up to 13 kb
- Optimum activity at 42°C
- Active up to 50°C
- Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)
|First strand cDNA synthesis for RT-PCR and real-time RT-PCR; Synthesis of cDNA for cloning and expression; Generation of labeled cDNA probes for microarrays; DNA labeling (3)|
|RevertAid Reverse Transcriptase|