Thermo Scientific™ PstI
The PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI).
Manufacturer: Thermo Scientific™ ER0612
Lambda DNA digested with PstI, 0.7% agarose, 28 cleavage sites
conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
5'..C T G C A▵G...3'
3'..G▵A C G T C...5'
Conditions for 100% Activity
- 1X Buffer O:50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSA
- Incubate at 37°C
- PstI is supplied in: 10mM Tris-HCl (pH7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerol
Ligation and Recleavage
- After 50-fold overdigestion with PstI, more than 95% of the DNA fragments can be ligated and recut
- Dam: never overlaps —no effect
- Dcm: never overlaps —no effect
- CpG: never overlaps —no effect
- EcoKI: never overlaps —no effect
- EcoBI: never overlaps —no effect
Digestion of Agarose-embedded DNA
- Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours
- Alw21I, ApaI, BseSI, Eco24I, Mph1103I, PstI, SacI, SdaI
Conditions of high pH, low salt, high glycerol, 8% DMSO can cause star activity (Malyguine, E., et al., Gene, 8, 163-177, 1980). Surrounding sequences: the presence of adjacent runs of G-C base pairs confers significant resistance to cleavage (Armstrong, K. and Bauer, W.R., NAR, 10, 993-1007, 1982). 100% dUTP incorporation at the recognition site reduces PstI cleavage to 25% (Glenn, T.C., et al., Biotechniques, 17, 1086-1090, 1994). PstI will not cut AGCTGCAG when methylated by AluI methyltransferase.
|5 x 3,000 U|