Cut at G^AWTC sites with PfeI (TfiI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: TfiI).
Supplier: Thermo Scientific™ ER1781
Lambda DNA digested with PfeI (TfiI), 0.7% agarose, 87 cleavage sites
conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Highlights
| 10 U/μL | |
| PfeI (TfiI) | |
| Yes | |
| No | |
| Restriction Enzyme |
| CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive | |
| 10X Buffer O | |
| 37°C | |
| 500 units | |
| Traditional Cloning |
For Research Use Only. Not for use in diagnostic procedures.
