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  5. Thermo Scientific™ MboII (5 U/μL)

Thermo Scientific™ MboII (5 U/μL)

Numéro de catalogue. FERER0821
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300 units
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FERER0821 300 units
FERER0822 1,500 units
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Numéro de catalogue. FERER0821 Fournisseur Thermo Scientific™ Code fournisseur ER0821
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Description

The MboII restriction enzyme recognizes GAAGA(8/7)^ sites and cuts best at 37°C in B buffer.

Lambda DNA (dam-) digested with MboII, 1.4% agarose, 130 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...G A A G A (N)8▵...3'

3'...C T T C T (N)7▵...5'

Conditions for 100% Activity:

  • 1X Buffer B: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • MboII is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: may overlap —blocked
  • Dcm: never overlaps —no effect
  • CpG: may overlap —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —no effect

Note:

Assayed using lambda DNA (dam) (#SD0021). MboII is blocked by overlappingdam methylation. To avoiddam methylation, use adam,dcm- strain such as GM2163 (#M0099). Greater than 15-fold overdigestion with MboII may result in star activity. MboII produces DNA fragments that have a single-base 3 extension which are more difficult to ligate than blunt-ended fragments. MboII may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.

Spécifications

Concentration 5 U/μL
Methylation Sensitivity Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Enzyme MboII
Compatible Buffer 10X Buffer B
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Type IIS RE Yes
Quantity 300 units
Product Type Restriction Enzyme
Research Category Scarless Cloning

For Research Use Only. Not for use in diagnostic procedures.