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Thermo Scientific™ HphI (10 U/μL)
The HphI restriction enzyme recognizes GGTGA(8/7)^ sites and cuts best at 37°C in B buffer (Isoschizomers: AsuHPI).
$90.54 - $403.09
Specifications
| Concentration | 10 U/μL |
|---|---|
| Methylation Sensitivity | Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive |
| Enzyme | HphI |
| Compatible Buffer | 10X Buffer B |
| Sensitive to Heat Inactivation | Yes |
Description
Lambda DNA (dam- digested with HphI, 1.4% agarose, 168 cleavage sites
conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Highlights
- Superior quality -stringent quality control and industry leading manufacturing process
- Convenient color-coded Five Buffer System
- Includes universal Tango buffer for double-digestions
- BSA premixed in reaction buffers
- Wide selection of restriction endonuclease specificities
Specifications
| 10 U/μL | |
| HphI | |
| Yes | |
| Yes | |
| Scarless Cloning |
| Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive | |
| 10X Buffer B | |
| 37°C | |
| Restriction Enzyme |
For Research Use Only. Not for use in diagnostic procedures.