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  5. Thermo Scientific™ GeneJET Gel Extraction Kit

Thermo Scientific™ GeneJET Gel Extraction Kit

Catalog No. ferk0692
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Description

Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.

Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.

The kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column. The kit can be used to purify DNA fragments from 25 bp to 20 kb in size with recovery rates up to 95%. Each GeneJET purification column has a binding capacity of up to 25 μg of DNA and can process up to 1 g of agarose gel. The entire procedure takes just 15 minutes, and the isolated DNA is ready to use in all common downstream applications, including ligation, restriction digestion, PCR, sequencing, and labeling.

Highlights

  • Highly efficient: up to 95% recoveries in the range of 100 bp to 10 kb
  • Fast: procedure takes only 15 minutes
  • Convenient: spin columns are capped and assembled with collection tubes

Applications

Fast and efficient extraction of high purity DNA fragments ideal for use in all conventional molecular biology procedures including:

  • FastDigest or conventional restriction digestion
  • Automated fluorescent or radioactive sequencing
  • PCR
  • In situ hybridization
  • Labeling
  • Cloning

Specifications

Column Type Spin Column
Content And Storage For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Isolation Technology Silica Spin Column
Sample Type Agarose gels
Elution Volume 50 μL
Final Product Type DNA
For Use With (Application) PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput Compatibility Not High-throughput Compatible (Manual)
Quantity 250 Preps
Shipping Condition Room Temperature
Test Time 15 min.
Yield 25 μg (Binding capacity)
Starting Material Amount ≤1 g
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Frequently Asked Questions (FAQs)

Can I use water instead of Elution Buffer in the elution step of GeneJET and MagJET nucleic acid purification kits?

We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.
Can I use a vacuum manifold with GeneJET kits?

The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.
Can I use GeneJET Gel Extraction Kit for PCR clean-up?

The GeneJET Gel Extraction Kit is designed for gel extraction but can also be used for PCR clean-up by following the protocol from the GeneJET PCR Purification Kit. The components in the GeneJET Gel Extraction Kit and GeneJET PCR Purification Kit are very similar and can be used interchangeably. The exact composition of solutions in the kits is proprietary.

For a 3-in-1 kit for purification of PCR products from enzymatic reaction mixtures and agarose gels, we recommend using the GeneJET Gel Extraction and DNA Cleanup Micro Kit (Cat. Nos. K0831/2).

At what temperature should I perform centrifugation steps in the GeneJET kits?

We recommend performing centrifugation steps at room temperature when the temperature is not specified.
Are GeneJET spin columns or MagJET magnetic beads available separately?

No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.
Do you offer the GeneJET Purification Columns included in the GeneJET Gel Extraction Kit as a stand-alone product?

The wash buffers and bindings buffers for the GeneJet kits are in most cases available separately. Unfortunately, the purification columns for all GeneJet kits including the GeneJET Gel Extraction Kit are not available separately. They can only be acquired as part of the whole kit itself.

For Research Use Only. Not for use in diagnostic procedures.