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Thermo Scientific™ FastDigest BamHI

Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

$73.99 - $163.99

Specifications

Methylation Sensitivity Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Enzyme BamH I
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
FERFD0054
View Documents Promotion Details
Thermo Scientific™
FD0054
800 μL
Each for $73.99
 
FERFD0055
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Thermo Scientific™
FD0055
5 x 500 μL
Each for $163.99
 
Description

Description

FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Highlights

  • 100% activity of all FastDigest enzymes in the universal buffer
  • 100% buffer compatibility with downstream applications
  • Complete digestion in 5-15 minutes
  • Direct loading on gels
  • No star activity
  • 176 FastDigest enzymes available
Specifications

Specifications

Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
10X FastDigest Buffer/FastDigest Green Buffer
37°C
Restriction Enzyme
FastDigest
BamH I
Yes
No
Traditional cloning
SDS
Product Certifications