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Thermo Scientific™ DraI, HC (50 U/μL)
Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.
Supplier: Thermo Scientific™ ER0223
Description
Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.
5'...T T T▵A A A...3'
3'...A A A▵T T T...5'
Conditions for 100% Activity:
- 1X Buffer Tango™:
- 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
- Incubate at 37°C
Storage Buffer:
- DraI is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerol
Ligation and Recleavage:
- After 50-fold overdigestion with DraI, more than 95% of the DNA fragments can be ligated and recut
Methylation Effects:
- Dam: never overlaps — no effect
- Dcm: never overlaps — no effect
- CpG: never overlaps — no effect
- EcoKI: may overlap — blocked
- EcoBI: never overlaps — no effect
Digestion of Agarose-embedded DNA:
- Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours
Specifications
| 50 U/μL | |
| Dra I | |
| Yes | |
| No | |
| Restriction Enzyme |
| Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive | |
| 10X Buffer Tango | |
| 37°C | |
| 7500 units | |
| Traditional Cloning |
For Research Use Only. Not for use in diagnostic procedures.