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Thermo Scientific™ CpoI (RsrII) (10 U/μL)

The CpoI (RsrII) restriction enzyme recognizes CG^GWCCG sites and cuts best at 37°C in Tango buffer (Isoschizomers: CspI, Rsr2I, RsrII).

Supplier: Thermo Scientific™ ER0741

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Catalog No. FERER0741

$147.00 / Each

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Description

Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...C GâµG W C C G...3'

3'...G C C W GâµG C...5'

Conditions for 100% Activity:

1X Buffer Tango:33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
Incubate at 37°C

Storage Buffer:

CpoI is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

After 50-fold overdigestion with CpoI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

Dam: never overlaps-no effect
Dcm: never overlaps-no effect
CpG: completely overlaps-blocked
EcoKI: never overlaps-no effect
EcoBI: never overlaps-no effect

Compatible Ends:

Cfr13I, Eco47I, Psp5II, SanDI

Specifications

Concentration	10 U/μL
Enzyme	CpoI (RsrII)
Sensitive to Heat Inactivation	Yes
Type IIS RE	No
Product Type	Restriction Enzyme

Methylation Sensitivity	CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer	10X Buffer Tango
Optimal Reaction Temperature	37°C
Quantity	200 units
Research Category	Traditional Cloning

Product Suggestions Documents

Documents

Product Information

Manuals

User Guide: CpoI (RsrII), 10 U/uL, 1000U Version: Jan. 2015 File size: 61.9 kB

User Guide: CpoI (RsrII), 10 U/uL, 200U Version: Jan. 2015 File size: 49.5 kB

For Research Use Only. Not for use in diagnostic procedures.