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Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).

Manufacturer:  Thermo Scientific™ B49

Catalog No. FERB49



Description

Description

Use the Thermo Scientific 50X TAE Buffer (Tris-acetate-EDTA) for electrophoresis of nucleic acids in agarose and polyacrylamide gels. You can use this buffer for both genomic and large supercoiled DNA, and you can also use this as both a running and a gel preparation buffer.

Usage Recommendations

  • Use fresh 1X TAE both for the gel and for the electrophoresis run
  • To prepare 1X TAE buffer add 20ml of 50X TAE buffer to 980ml of deionized water and mix well

Quality Control

  • The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests

1X Composition

  • 40mM Tris
  • 20mM acetic acid
  • 1mM EDTA
  • pHof 50X TAE: 8.4

Recommended for:

Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for resolution of RNA and DNA fragments l.

Note:

TAE buffer has a relatively low buffering capacity, therefore periodic replacement of the buffer during prolonged electrophoresis is recommended.

Specifications

Specifications

50 X
Acrylamide Gels, Agarose Gels
SDS
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