Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)
Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).
Manufacturer: Thermo Scientific™ B49
Use the Thermo Scientific 50X TAE Buffer (Tris-acetate-EDTA) for electrophoresis of nucleic acids in agarose and polyacrylamide gels. You can use this buffer for both genomic and large supercoiled DNA, and you can also use this as both a running and a gel preparation buffer.
- Use fresh 1X TAE both for the gel and for the electrophoresis run
- To prepare 1X TAE buffer add 20ml of 50X TAE buffer to 980ml of deionized water and mix well
- The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests
- 40mM Tris
- 20mM acetic acid
- 1mM EDTA
- pHof 50X TAE: 8.4
Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for resolution of RNA and DNA fragments l.
TAE buffer has a relatively low buffering capacity, therefore periodic replacement of the buffer during prolonged electrophoresis is recommended.
|Acrylamide Gels, Agarose Gels|