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Thermo Scientific™ TBE Buffer (Tris-borate-EDTA) (10X)

Catalog No. FERB52
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FERB52 1 L
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Catalog No. FERB52 Supplier Thermo Scientific™ Supplier No. B52
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Description

Perform electrophoresis of nucleic acids in agarose and polyacrylamide gels using Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA).

TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels, but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer (Tris Acetate-EDTA buffer, 10X powder, sc-296647) is recommended when looking at enzymatic applications for the DNA sample.

Usage Recommendations

  • Use fresh 1X TBE both for the gel and for the electrophoresis run
  • To prepare 1X TBE buffer, add 100mL of 10X TBE buffer to 900mL of deionized water and mix well

Quality Control

  • The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests

1X Composition

  • 89mM Tris, 89mM boric acid, 2mM EDTA, pH of 10X TBE: 8.3

Recommended for:

Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for electrophoresis of RNA and DNA fragme.

Note:

Double-stranded linear nucleic acid molecules migrate about 10% slower in TBE buffer than in TAE buffer.

Specifications

Concentration 10X
Quantity 1 L
Product Type TBE Buffer

Frequently Asked Questions (FAQs)

For the purpose of recovering and solubilizing nucleic acids in alcohol, can TAE running buffer be used instead of TBE running buffer when running a pre-cast TBE gel?

A TAE running buffer used in conjunction with a TBE gel would create high current and may lead to gel hydrolysis. Also, the acetate ion in TAE migrates faster than the borate ion in TBE buffer, and can create fuzzy bands. It is possible to try soaking the TBE gel (run in TBE buffer) in the TAE buffer following the run and then eluting from that. Since nucleic acids diffuse out of the gel relatively slowly, this may work.

For Research Use Only. Not for use in diagnostic procedures.