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Invitrogen™ Human FcGR2BC (CD32 B/C) ELISA Kit 
ELISA
Supplier: Invitrogen™ EHFCGR2B
Description
The Human FcGR2BC (CD32 B/C) ELISA quantitates Hu FcGR2BC in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FcGR2BC. Principle of the method The Human FcGR2BC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.Specifications
| P31994,P31995 | |
| 85 pg/mL | |
| Solid-phase sandwich ELISA | |
| ELISA | |
| Plasma, Serum, Supernatant | |
| 96 Assays | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 2212, 2213, 9103 | |
| <12% | |
| Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| 96 Tests | |
| Immunology | |
| 2°C to 8°C | |
| CD32 | |
| 4 hrs 45 mins |
| 78.13 to 5000 pg/mL | |
| 85 pg/mL to 5000 pg/mL | |
| HRP | |
| Other Proteins | |
| This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. | |
| Human | |
| Colorimetric Microplate Reader | |
| CD32,CD32B,FCG2,FCGR2,IGFR2,CD32,FCG2,CD32C,CDW32,IGFR2,FCRIIC | |
| <10 | |
| HRP | |
| RUO | |
| Plasma 2.5 μL, Serum 2.5 μL, Supernatant 100 μL | |
| RP11-474I16.2, CD32, CD32B, FCG2, FCGR2, IGFR2 | |
| 1 hrs 20 mins |