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Thermo Scientific™ FastDigest™ NdeI

The FastDigest NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FauNDI).

$65.10 - $148.05

Specifications

Components 10X FastDigest Buffer and 10X FastDigest Green Buffer
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Products
Catalog Number Mfr. No. Quantity Price Quantity    

FERFD0584

 
thermo scientific™
FD0584
300µL Each for $65.10

FERFD0585

 
thermo scientific™
FD0585
2 x 500µL Each for $148.05
Description & Specifications

Specifications

Components 10X FastDigest Buffer and 10X FastDigest Green Buffer

Lambda DNA digested with NdeI, 0.7% agarose, 7 cleavage sites

FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Highlights

  • 100% activity of all FastDigest enzymes in the universal buffer
  • 100% buffer compatibility with downstream applications
  • Complete digestion in 5-15 minutes
  • Direct loading on gels
  • No star activity
  • 176 FastDigest enzymes available