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Invitrogen™ F(ab)-Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor™ Plus 488

Catalog No. 5001248851
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5001248851 250 μL
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Catalog No. 5001248851 Supplier Invitrogen™ Supplier No. SA510394AFP488

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Description

F(ab)-Rabbit Recombinant Superclonal Secondary Antibody

F(ab)-Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody detects both the Fab2 and Fc fragments of Goat IgG. It showed no cross-reactivity to various species immunoglobulins tested. This Fab fragment antibody contains a hexa-histidine tag, is expressed in vitro and purified as a recombinant construct. The advantages of recombinant antibodies include better specificity, animal origin-free formulation, and lot-to-lot consistency. F(ab) fragments offer several advantages over full-length IgGs due to their smaller molecular size (~50 kDa vs. ~150 kDa), such as improved tissue penetration. The absence of the Fc region reduces nonspecific binding and background staining in Fc receptor-expressing cells and tissues, while also enabling increased flexibility in multiplexing assays. Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Goat IgG (H+L)
Applications Western Blot, Immunocytochemistry
Classification Recombinant Superclonal
Concentration 1 mg/mL
Conjugate Alexa Fluor Plus 488
Formulation Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.3-7.3
Host Species Rabbit
Immunogen Purified Goat IgG.
Purification Method Affinity chromatography
Quantity 250 μL
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Goat
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Secondary Antibody
Form Liquid
Isotype IgG F(ab)
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