Invitrogen™ F(ab)-Goat anti-Rabbit IgG Fc Superclonal™ Secondary Antibody, Alexa Fluor™ Plus 488

F(ab)-Goat Recombinant Superclonal Secondary Antibody

F(ab)-Goat anti-Rabbit IgG Fc Recombinant Secondary Antibody, Alexa Fluor™ Plus 488 is specific to Rabbit IgG Fc fragment. It showed no cross-reactivity to various species immunoglobulins tested except for <10% reactivity towards Human IgG, Monkey IgG and Donkey IgG. This Fab fragment antibody is expressed in vitro and purified as a recombinant construct. It is also engineered to deliver enhanced stability and sensitivity. The advantages of recombinant antibodies include-better specificity, animal origin-free formulation, and lot-to-lot consistency. F(ab) fragments offer several advantages over full-length IgGs due to their smaller molecular size (~50 kDa vs. ~150 kDa), such as improved tissue penetration. The absence of the Fc region reduces nonspecific binding and background staining in Fc receptor-expressing cells and tissues, while also enabling increased flexibility in multiplexing assays. Alexa Fluor™ Plus dyes have been chemically optimized to provide higher signal to noise ratios and minimized cross-reactivity to help detect low-abundance targets in rare or precious samples. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.