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Thermo Scientific™ EZ-Link™ Sulfo NHS-SS Biotinylation Kit
Biotinylate protein amines with this cleavable, mid-length (24.3A), water-soluble, NHS-ester biotinylation reagent containing a cleavable disulfide bond in the spacer arm.
Supplier: Thermo Scientific™ 21445
Description
Thermo Scientific™ EZ-Link Sulfo-NHS-SS-Biotin enables simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution.
Highlights:
- Cleavable analog of Sulfo-NHS-LC-Biotin
- Can remove biotin label by cleaving spacer disulfide bond with reducing agents, such as DTT
- Ideal for affinity purification experiments that require efficient elution from immobilized avidin supports (cleaving disulfide bond releases proteins)
- Long spacer arm reduces steric hindrance for binding interactions
Specifications
| EZ-Link Sulfo-NHS-SS-Biotinylation Kit | |
| Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody • EZ-Link Sulfo-NHS-SS-Biotin, 25 mg • PBS Pack (makes 500 mL), 1 pack • Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns • HABA Solution, 1 mL • Avidin, 10 mg Store avidin at -20°C. Store biotin reagent desiccated at 4°C. Store remaining kit components at 4°C. |
|
| EZ-Link™ | |
| Biotin |
| Sulfo-NHS-SS Biotinylation Kit | |
| 1 to 10 mg | |
| Water | |
| Antibodies, Proteins |
For Research Use Only. Not for use in diagnostic procedures.