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Thermo Scientific™ EZ-Link™ Sulfo NHS-SS Biotinylation Kit

Catalog No. PI21445
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PI21445 10 Reactions
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Catalog No. PI21445 Supplier Thermo Scientific™ Supplier No. 21445
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Description

Biotinylate protein amines with this cleavable, mid-length (24.3A), water-soluble, NHS-ester biotinylation reagent containing a cleavable disulfide bond in the spacer arm.

Thermo Scientific™ EZ-Link Sulfo-NHS-SS-Biotin enables simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution.

Highlights:

  • Cleavable analog of Sulfo-NHS-LC-Biotin
  • Can remove biotin label by cleaving spacer disulfide bond with reducing agents, such as DTT
  • Ideal for affinity purification experiments that require efficient elution from immobilized avidin supports (cleaving disulfide bond releases proteins)
  • Long spacer arm reduces steric hindrance for binding interactions

Specifications

Description EZ-Link Sulfo-NHS-SS-Biotinylation Kit
Product Type Sulfo-NHS-SS Biotinylation Kit
Quantity 10 Reactions
Content And Storage Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
• EZ-Link Sulfo-NHS-SS-Biotin, 25 mg
• PBS Pack (makes 500 mL), 1 pack
• Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns
• HABA Solution, 1 mL
• Avidin, 10 mg

Store avidin at -20°C. Store biotin reagent desiccated at 4°C. Store remaining kit components at 4°C.
Labeling Scale 1 to 10 mg
Product Line EZ-Link
Solubility Water
Label or Dye Biotin
Labeling Target Antibodies, Proteins

Frequently Asked Questions (FAQs)

Is there a cleavable analog of Sulfo-NHS-LC-Biotin?

Yes, we offer Sulfo-NHS-SS-Biotin (Cat. Nos. 21331, 21328). It has a disulfide bond in the spacer arm that can be cleaved with DTT or other reducing agents, thereby releasing the biotin tag from the previously labeled protein. This product is also available in kit format (Cat. Nos. 21945, 21445).
How do the EZ-Link Micro Biotinylation Kits differ from the standard biotinylation kits?

The standard biotinylation kits contain components for labeling 1-10 mg of protein, and the Micro Biotinylation Kits are structured for labeling 50-200 µg of protein in 200-700 mL. The Micro kits contain the biotinylation reagents in small, individual packages and do not include components to perform the HABA assay.
Can you provide the shelf-life for EZ-Link Sulfo NHS-SS Biotinylation Kit?

EZ-Link Sulfo NHS-SS Biotinylation Kit is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

For Research Use Only. Not for use in diagnostic procedures.