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Thermo Scientific™ EZ-Link™ Sulfo NHS-SS Biotinylation Kit

Catalog No. PI21445
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10 Reactions
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PI21445 10 Reactions
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Catalog No. PI21445 Supplier Thermo Scientific™ Supplier No. 21445
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Description

Biotinylate protein amines with this cleavable, mid-length (24.3A), water-soluble, NHS-ester biotinylation reagent containing a cleavable disulfide bond in the spacer arm.

Thermo Scientific™ EZ-Link Sulfo-NHS-SS-Biotin enables simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution.

Highlights:

  • Cleavable analog of Sulfo-NHS-LC-Biotin
  • Can remove biotin label by cleaving spacer disulfide bond with reducing agents, such as DTT
  • Ideal for affinity purification experiments that require efficient elution from immobilized avidin supports (cleaving disulfide bond releases proteins)
  • Long spacer arm reduces steric hindrance for binding interactions

Specifications

Description EZ-Link Sulfo-NHS-SS-Biotinylation Kit
Product Type Sulfo-NHS-SS Biotinylation Kit
Quantity 10 Reactions
Content And Storage Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
• EZ-Link Sulfo-NHS-SS-Biotin, 25 mg
• PBS Pack (makes 500 mL), 1 pack
• Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns
• HABA Solution, 1 mL
• Avidin, 10 mg

Store avidin at -20°C. Store biotin reagent desiccated at 4°C. Store remaining kit components at 4°C.
Labeling Scale 1 to 10 mg
Product Line EZ-Link
Solubility Water
Label or Dye Biotin
Labeling Target Antibodies, Proteins

For Research Use Only. Not for use in diagnostic procedures.