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Thermo Scientific™ EZ-Link™ Micro Sulfo-NHS-LC-Biotinylation Kit

Catalog No. PI21935
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Quantity:
8 Reactions
Catalog No. Quantity
PI21935 8 Reactions
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Catalog No. PI21935

Supplier: Thermo Scientific™ 21935

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Description

Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Thermo Scientific™ EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Highlights:

  • Water-soluble analog of NHS-LC-Biotin
  • Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phosphate buffers
  • Negative charge of -SO3 group keeps biotinylation localized at the cell surface
  • Creates an amide bond
U.S. Patents 5,892,628 and 5,872,261.

Specifications

Description EZ-Link Micro Sulfo-NHS-LC-Biotinylation Kit
Product Type Micro Sulfo-NHS-LC-Biotinylation Kit
Quantity 8 Reactions
Content And Storage Sufficient For: 8 labeling reactions, each with 0.05–0.2 mg of antibody
• EZ-Link Sulfo-NHS-LC-Biotin, 8 x 1 mg
• PBS Pack (makes 500 mL), 1 pack
• Zeba Spin Desalting Columns (7K MWCO), 2 mL, 10 columns

Store biotin reagent at -20°C. Store remaining kit components at 4°C.
Labeling Scale 100 μg
Product Line EZ-Link
Solubility Water
Label or Dye Biotin
Labeling Target Antibodies, Proteins

For Research Use Only. Not for use in diagnostic procedures.