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Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit

Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Manufacturer:  Thermo Scientific™ 0021435

Catalog No. PI21435



Description

Description

Thermo Scientific™ EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Highlights:

  • Water-soluble analog of NHS-LC-Biotin
  • Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phosphate buffers
  • Negative charge of -SO3 group keeps biotinylation localized at the cell surface
  • Creates an amide bond
U.S. Patents 5,892,628 and 5,872,261.
Specifications

Specifications

EZ-Link Sulfo-NHS-LC-Biotinylation Kit
Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
• EZ-Link Sulfo-NHS-LC-Biotin, 25 mg
• PBS Pack (makes 500 mL), 1 pack
• Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns
• HABA Solution, 1 mL
• Avidin, 10 mg

Store biotin and avidin reagents at -20°C. Store remaining kit components at 4°C.
EZ-Link™
Biotin
Sulfo-NHS-LC-Biotinylation Kit
1 to 10 mg
Water
Antibodies (General), Proteins (General)
SDS
Documents
Product Certifications
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