missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Crosslinking, Labeling and Protein Modification
  5. Protein and Antibody Labeling Kits
  6. PI21435

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit

Catalog No. PI21435
Change view
Click to view available options
Quantity:
10 reactions
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PI21435 10 reactions
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. PI21435 Supplier Thermo Scientific™ Supplier No. LSG21435
Only null left

Description

Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Thermo Scientific™ EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Highlights:

  • Water-soluble analog of NHS-LC-Biotin
  • Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phosphate buffers
  • Negative charge of -SO3 group keeps biotinylation localized at the cell surface
  • Creates an amide bond
U.S. Patents 5,892,628 and 5,872,261.

Specifications

Description EZ-Link Sulfo-NHS-LC-Biotinylation Kit
Product Type Sulfo-NHS-LC-Biotinylation Kit
Quantity 10 reactions
Content And Storage Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
• EZ-Link Sulfo-NHS-LC-Biotin, 25 mg
• PBS Pack (makes 500 mL), 1 pack
• Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns
• HABA Solution, 1 mL
• Avidin, 10 mg

Store biotin and avidin reagents at -20°C. Store remaining kit components at 4°C.
Labeling Scale 1 to 10 mg
Product Line EZ-Link
Solubility Water
Label or Dye Biotin
Labeling Target Antibodies, Proteins

Frequently Asked Questions (FAQs)

In the EZ-Link Sulfo-NHS-LC-Biotinylation kit, what groups does Sulfo-NHS-LC-Biotin react with for biotinylation?

The NHS ester on this biotinylation reagent reacts with primary amines. In proteins and peptides, these occur at the amino terminus and the epsilon amine (side chain) of lysine residues.
How do the EZ-Link Micro Biotinylation Kits differ from the standard biotinylation kits?

The standard biotinylation kits contain components for labeling 1-10 mg of protein, and the Micro Biotinylation Kits are structured for labeling 50-200 µg of protein in 200-700 mL. The Micro kits contain the biotinylation reagents in small, individual packages and do not include components to perform the HABA assay.
Can you provide the shelf-life for EZ-Link Sulfo-NHS-LC-Biotinylation Kit?

EZ-Link Sulfo-NHS-LC-Biotinylation Kit is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
Is there any advantage of choosing a Sulfo-NHS biotin with a longer spacer arm than EZ-Link Sulfo NHS Biotin?

Sometimes. The biotin has to insert 9 angstrom into the pocket of avidin, streptavidin or NeutrAvidin to bind. If steric hindrance may be a problem, using a biotinylation reagent with a longer spacer arm (Sulfo-NHS-LC Biotin or Sulfo-NHS-LC-LC Biotin) may result in greater purification or deteciton.
Why would I chose a Sulfo-NHS Biotin over its non-sulfonated counterpart?

The sodium sulfonate group lends water solubility to the reagent.
Note: The actual biotin moiety that is added is identical between NHS and Sulfo-NHS biotins that vary only in the addition of the sodium sulfonate on the leaving group. Researchers preferring a more water soluble biotin be added to their molecule should consider a PEGylated version of biotin.

For Research Use Only. Not for use in diagnostic procedures.