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Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin and Labeling Kits

Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Manufacturer: thermo scientific™  PI21435

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Catalog No. PI21435

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Description & Specifications


Chemical Reactivity Amine
Water Soluble Yes
Sufficient For 10 labeling reactions, each with 1 to 10mg of antibody
Storage Requirements -20°C with desiccant
Kit Contents Avidin, 10mg
Description EZ-Link Sulfo-NHS-LC-Biotinylation Kit
PEGylated No
Spacer Arm Length 22.4
Cleavable No
Quantity 10 rxn kit
Membrane Permeable No
Formulation Multi-component kit with Sulfo-NHS-LC-Biotin
Molecular Weight 556.59

Thermo Scientific™ EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.


  • Water-soluble analog of NHS-LC-Biotin
  • Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phosphate buffers
  • Negative charge of -SO3 group keeps biotinylation localized at the cell surface
  • Creates an amide bond
U.S. Patents 5,892,628 and 5,872,261.