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Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit

Catalog No. PI21435
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10 reactions
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Catalog No. Quantity
PI21435 10 reactions
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Catalog No. PI21435 Supplier Thermo Scientific™ Supplier No. LSG21435
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Description

Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Thermo Scientific™ EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Highlights:

  • Water-soluble analog of NHS-LC-Biotin
  • Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phosphate buffers
  • Negative charge of -SO3 group keeps biotinylation localized at the cell surface
  • Creates an amide bond
U.S. Patents 5,892,628 and 5,872,261.

Specifications

Description EZ-Link Sulfo-NHS-LC-Biotinylation Kit
Product Type Sulfo-NHS-LC-Biotinylation Kit
Quantity 10 reactions
Content And Storage Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
• EZ-Link Sulfo-NHS-LC-Biotin, 25 mg
• PBS Pack (makes 500 mL), 1 pack
• Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns
• HABA Solution, 1 mL
• Avidin, 10 mg

Store biotin and avidin reagents at -20°C. Store remaining kit components at 4°C.
Labeling Scale 1 to 10 mg
Product Line EZ-Link
Solubility Water
Label or Dye Biotin
Labeling Target Antibodies, Proteins

For Research Use Only. Not for use in diagnostic procedures.