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Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin
Description
Thermo Scientific™ EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
- Protein labeling - biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
- Cell surface labeling - biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
- Amine-reactive - reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
- Soluble - charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
- Irreversible - forms permanent amide bonds; spacer arm cannot be cleaved
- Medium length - spacer arm (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass
Specifications
Specifications
| Product Type | Sulfo-NHS-LC-Biotin |
| Quantity | 10 x 1 mg |
| Content And Storage | Store desiccated at -20°C. |
| Labeling Method | Chemical Labeling |
| Shipping Condition | Qualified for Ambient or Wet Ice |
| Product Line | EZ-Link |
| Cell Permeability | Cell-Impermeant |
| Chemical Reactivity | Amine |
| Reactive Moiety | NHS Ester |
| Solubility | DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water |
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For Research Use Only. Not for use in diagnostic procedures.