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Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin
Description
Thermo Scientific™ EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
- Protein labeling - biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
- Cell surface labeling - biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
- Amine-reactive - reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
- Soluble - charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
- Irreversible - forms permanent amide bonds; spacer arm cannot be cleaved
- Medium length - spacer arm (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass
Specifications
Specifications
| Product Type | Sulfo-NHS-LC-Biotin |
| Quantity | 10 x 1 mg |
| Content And Storage | Store desiccated at -20°C. |
| Labeling Method | Chemical Labeling |
| Shipping Condition | Qualified for Ambient or Wet Ice |
| Product Line | EZ-Link |
| Cell Permeability | Cell-Impermeant |
| Chemical Reactivity | Amine |
| Reactive Moiety | NHS Ester |
| Solubility | DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water |
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Frequently Asked Questions (FAQs)
No, the EZ-Link Sulfo-NHS-LC-Biotin, No-Weigh Format (Cat. No. A39257) should not be dissolved in water for storage. We recommended storing EZ-Link Sulfo-NHS-LC-Biotin, No-Weigh Format at -20 degrees C with desiccant as it is very moisture sensitive. Furthermore, to avoid moisture condensation onto the product, equilibrate vial to room temperature before opening.
EZ-Link Sulfo-NHS-LC-Biotin is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (-20 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
EZ-Link Sulfo-NHS-LC-Biotin, No-Weigh Format is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (desiccated, at -20 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).
The No-Weigh product format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. This format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent. Please view additional information and the video at: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/no-weigh-packaging.html
For Research Use Only. Not for use in diagnostic procedures.