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Thermo Scientific™ EZ-Link™ Micro Sulfo-NHS-Biotinylation Kit

Biotinylate protein amines (e.g., lysines) with our shortest (13.5A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Manufacturer:  Thermo Scientific™ 0021925

Catalog No. PI21925



Description

Description

Label antibodies, proteins and other molecules that have primary amines with Thermo Scientific™ EZ-Link Sulfo-NHS-Biotin, a short-chain, water-soluble biotinylation reagent.

Highlights:

  • Shorter spacer arm than Sulfo-NHS-LC-Biotin
  • Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate, or phosphate buffers
  • Negative charge of -SO3 keeps biotinylation localized at the cell surface so reagent does not pass through cell membrane
  • Water solubility eliminates the need for harmful organic solvents
U.S. Patents 5,892,628 and 5,872,261.
Specifications

Specifications

EZ-Link Micro Sulfo-NHS-Biotinylation Kit
Sufficient For: 8 labeling reactions, each with 0.05–0.2 mg of antibody
• EZ-Link Sulfo-NHS-Biotin, 8 x 1 mg
• PBS Pack (makes 500 mL), 1 pack
• Zeba Spin Desalting Columns (7K MWCO), 2 mL, 10 columns

Store biotin reagent at -20°C. Store remaining kit components at 4°C.
EZ-Link™
Biotin
Antibodies (General), Proteins (General)
Micro Sulfo-NHS-Biotinylation Kit
100 μg
Water
Pegylated
SDS
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Product Certifications
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