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Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin and Labeling Kits

Biotinylate protein amines (e.g., lysines) with our shortest (13.5A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Manufacturer: thermo scientific™  21925*

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Catalog No. PI21925

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Description & Specifications


Molecular Weight 443.43
Cleavable No
Formulation Multi-component kit with Sulfo-NHS-Biotin
Description EZ-Link Micro Sulfo-NHS-Biotinylation Kit
Storage Requirements -20°C with desiccant
Kit Contents EZ-Link Sulfo-NHS-Biotin, 8 x 1mg
Membrane Permeable No
Quantity 8 rxn kit
PEGylated No
Water Soluble Yes
Sufficient For 8 labeling reactions, each with 0.05-0.2mg of antibody
Chemical Reactivity Amine
Spacer Arm Length 13.5

Label antibodies, proteins and other molecules that have primary amines with Thermo Scientific™ EZ-Link Sulfo-NHS-Biotin, a short-chain, water-soluble biotinylation reagent.


  • Shorter spacer arm than Sulfo-NHS-LC-Biotin
  • Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate, or phosphate buffers
  • Negative charge of -SO3 keeps biotinylation localized at the cell surface so reagent does not pass through cell membrane
  • Water solubility eliminates the need for harmful organic solvents
U.S. Patents 5,892,628 and 5,872,261.