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Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin and Labeling Kits

Biotinylate protein amines (e.g., lysines) with our shortest (13.5A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Manufacturer: thermo scientific™  21425

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Catalog No. PI21425

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Description & Specifications


Kit Contents
  • EZ-Link Sulfo-NHS-Biotin, 25mg
  • PBS Pack (makes 500mL), 1 pack
  • Zeba Spin Desalting Columns (7K MWCO), 5mL, 10 columns
  • HABA Solution, 1mL
  • Avidin, 10mg
Membrane Permeable No
Quantity 10 rxn kit
Formulation Multi-component kit with Sulfo-NHS-Biotin
Storage Requirements -20°C with desiccant
Water Soluble Yes
Description EZ-Link Sulfo-NHS-Biotinylation Kit
Cleavable No
Spacer Arm Length 13.5
Molecular Weight 443.43
PEGylated No
Sufficient For 10 labeling reactions, each with 1 to 10mg of antibody
Chemical Reactivity Amine

Label antibodies, proteins and other molecules that have primary amines with Thermo Scientific™ EZ-Link Sulfo-NHS-Biotin, a short-chain, water-soluble biotinylation reagent.


  • Shorter spacer arm than Sulfo-NHS-LC-Biotin
  • Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate, or phosphate buffers
  • Negative charge of -SO3 keeps biotinylation localized at the cell surface so reagent does not pass through cell membrane
  • Water solubility eliminates the need for harmful organic solvents
U.S. Patents 5,892,628 and 5,872,261.