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Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotinylation Kit

Catalog No. PI21425
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PI21425 10 reactions
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Catalog No. PI21425 Supplier Thermo Scientific™ Supplier No. 21425
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Description

Biotinylate protein amines (e.g., lysines) with our shortest (13.5A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Label antibodies, proteins and other molecules that have primary amines with Thermo Scientific™ EZ-Link Sulfo-NHS-Biotin, a short-chain, water-soluble biotinylation reagent.

Highlights:

  • Shorter spacer arm than Sulfo-NHS-LC-Biotin
  • Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate, or phosphate buffers
  • Negative charge of -SO3 keeps biotinylation localized at the cell surface so reagent does not pass through cell membrane
  • Water solubility eliminates the need for harmful organic solvents
U.S. Patents 5,892,628 and 5,872,261.

Specifications

Description EZ-Link Sulfo-NHS-Biotinylation Kit
Product Type Sulfo-NHS-Biotinylation Kit
Quantity 10 reactions
Content And Storage Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
• EZ-Link Sulfo-NHS-Biotin, 25 mg
• PBS Pack (makes 500mL), 1 pack
• Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns
• HABA Solution, 1 mL
• Avidin, 10 mg

Store avidin and vial of biotin reagent (with desiccant) at -20°C. Store remaining kit components at 4°C.
Labeling Scale 1 to 10 mg
Product Line EZ-Link
Solubility Water
Label or Dye Biotin
Labeling Target Antibodies, Proteins

For Research Use Only. Not for use in diagnostic procedures.