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Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotinylation Kit
Biotinylate protein amines (e.g., lysines) with our shortest (13.5A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.
Supplier: Thermo Scientific™ 21425
Description
Label antibodies, proteins and other molecules that have primary amines with Thermo Scientific™ EZ-Link Sulfo-NHS-Biotin, a short-chain, water-soluble biotinylation reagent.
Highlights:
- Shorter spacer arm than Sulfo-NHS-LC-Biotin
- Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate, or phosphate buffers
- Negative charge of -SO3 keeps biotinylation localized at the cell surface so reagent does not pass through cell membrane
- Water solubility eliminates the need for harmful organic solvents
Specifications
| EZ-Link Sulfo-NHS-Biotinylation Kit | |
| Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody • EZ-Link Sulfo-NHS-Biotin, 25 mg • PBS Pack (makes 500mL), 1 pack • Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns • HABA Solution, 1 mL • Avidin, 10 mg Store avidin and vial of biotin reagent (with desiccant) at -20°C. Store remaining kit components at 4°C. |
|
| EZ-Link™ | |
| Biotin |
| Sulfo-NHS-Biotinylation Kit | |
| 1 to 10 mg | |
| Water | |
| Antibodies, Proteins |
For Research Use Only. Not for use in diagnostic procedures.