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  6. Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin

Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin

Catalog No. PIA39256
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50 mg
10 x 1 mg
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PIA39256 10 x 1 mg
PI21217 50 mg
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Catalog No. PIA39256 Supplier Thermo Scientific™ Supplier No. A39256
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Description

Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Label antibodies, proteins and other molecules that have primary amines with Thermo Scientific™ EZ-Link Sulfo-NHS-Biotin, a short-chain, water-soluble biotinylation reagent.

  • Protein labeling - biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
  • Cell surface labeling - biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
  • Amine-reactive - reacts with primary amines (-NH2), such as lysine side-chains or the amino-termini of polypeptides
  • Soluble - charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
  • Irreversible - forms permanent amide bonds; spacer arm cannot be cleaved
  • Very short - spacer arm (total length added to target) is 13.5 angstroms; it consists of the native biotin valeric acid group only.

Specifications

Kit Contents 10 x 1 mg
Product Type Sulfo-NHS-Biotin
Quantity 10 x 1 mg
Content And Storage Store desiccated at -20°C.
Labeling Method Chemical Labeling
Shipping Condition Qualified for Ambient or Wet Ice
Product Line EZ-Link
Cell Permeability Cell-Impermeant
Chemical Reactivity Amine
Reactive Moiety Sulfo-NHS Ester
Solubility DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
Label or Dye Biotin
Spacer Short
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Frequently Asked Questions (FAQs)

Can you provide the shelf-life for EZ-Link Sulfo-NHS-Biotin?

EZ-Link Sulfo-NHS-Biotin is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (-20 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
How does a product with "No-Weigh" format reduce my concern over reagent stability?

The No-Weigh product format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. This format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent. Please view additional information and the video at: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/no-weigh-packaging.html

For Research Use Only. Not for use in diagnostic procedures.