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Thermo Scientific™ EZ-Link™ NHS-SS-PEG4-Biotin

Catalog No. PI21442
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50 mg
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Catalog No. Quantity
PI21442 50 mg
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Catalog No. PI21442 Supplier Thermo Scientific™ Supplier No. 21442
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Description

Biotinylate protein amines with this cleavable, long (37.9A) NHS-ester biotinylation reagent containing a reducible disulfide bond in the pegylated, water-soluble spacer arm.

Thermo Scientific™ EZ-Link NHS-SS-PEG4-Biotin includes disulfide and polyethylene glycol (PEG) groups in its spacer arm to provide biotinylation that is cleavable and which enhances solubility.

Highlights:

  • Extended spacer arm reduces steric hindrance in binding to avidin, streptavidin and Thermo Scientific NeutrAvidin Protein
  • PEG spacer maintains water solubility of the modified compound
  • Disulfide bond in the spacer arm enables the biotin group to be cleaved from the protein by treatment with reducing agent
  • Useful in affinity purification experiments where it is necessary to recover biotin labeled protein from immobilized avidin, streptavidin or NeutrAvidin™ Supports

Specifications

Product Type NHS-SS-PEG4-Biotin
Quantity 50 mg
Content And Storage Store desiccated at -20°C. Shipped at ambient temperature.
Product Line EZ-Link
Cell Permeability Cell-Permeant
Chemical Reactivity Amine
Reactive Moiety Active Ester, Succinimidyl Ester, NHS Ester
Solubility DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
Label or Dye Biotin
Spacer Extra-long, Pegylated, Cleavable
Label Type Biotin & Analogs
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For Research Use Only. Not for use in diagnostic procedures.