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  6. Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin

Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin

Catalog No. PIA39259
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50 mg
10 x 2 mg
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Catalog No. Quantity
PIA39259 10 x 2 mg
PI21330 25 mg
PI21362 50 mg
PI21363 1 g
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Catalog No. PIA39259 Supplier Thermo Scientific™ Supplier No. A39259
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Description

Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.

Thermo Scientific™ EZ-Link NHS-PEG4-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.

  • Protein labeling - biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
  • Amine-reactive - reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
  • Pegylated - spacer arm contains a hydrophilic, 4-unit, polyethylene glycol (PEG) group
  • Enhances solubility - pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
  • Irreversible - forms permanent amide bonds; spacer arm cannot be cleaved
  • Long reach - spacer arm (total length added to target) is 29 angstroms; this reduces steric hindrance when binding to avidin molecules

Specifications

Product Type NHS-PEG4-Biotin
Quantity 10 x 2 mg
Content And Storage Store at -20°C.
Labeling Method Chemical Labeling
Shipping Condition Wet Ice
Product Line EZ-Link
Cell Permeability Cell-Impermeant
Chemical Reactivity Amine
Reactive Moiety NHS Ester
Solubility DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
Label or Dye Biotin
Spacer Pegylated, 29.0 Å
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Frequently Asked Questions (FAQs)

Can you provide the shelf-life for the EZ-Link NHS-SS-PEG4-Biotin?

The EZ-Link NHS-SS-PEG4-Biotin is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (-20 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
How does a product with "No-Weigh" format reduce my concern over reagent stability?

The No-Weigh product format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. This format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent. Please view additional information and the video at: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/no-weigh-packaging.html

For Research Use Only. Not for use in diagnostic procedures.