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  6. Thermo Scientific™ EZ-Link™ NHS-PEG12-Biotin, No-Weigh™ Format

Thermo Scientific™ EZ-Link™ NHS-PEG12-Biotin, No-Weigh™ Format

Catalog No. pi21312
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25 mg
10 x 1 mg
500 mg
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Catalog No. Quantity
PI21312 25 mg
PIA35389 10 x 1 mg
PI21313 500 mg
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Catalog No. PI21312 Supplier Thermo Scientific™ Supplier No. LSG21312
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Description

Biotinylate protein amines and maximize solubility of proteins with this very long (56.0A) NHS-ester biotinylation reagent containing a pegylated, water-soluble spacer arm.

Thermo Scientific™ EZ-Link NHS-PEG12-Biotin is very long, pegylated biotinylation reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.

Highlights:

  • Water-soluble polyethylene glycol (PEG) spacer arm increases solubility of labeled protein
  • Excellent purity, molecular weight uniformity and amine-reactivity
  • Long spacer arm reduces steric hindrance when binding to avidin molecules

Specifications

Product Type NHS-PEG12-Biotin
Quantity 25 mg
Content And Storage Store desiccated at -20°C. Shipped at ambient temperature.
Product Line EZ-Link
Cell Permeability Cell-Impermeant
Chemical Reactivity Amine
Reactive Moiety Active Ester, Succinimidyl Ester, NHS Ester
Solubility DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
Label or Dye Biotin
Spacer Extra-long, Pegylated
Label Type Biotin & Analogs
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Frequently Asked Questions (FAQs)

Can you provide the shelf-life for EZ-Link NHS-PEG12-Biotin?

EZ-Link NHS-PEG12-Biotin is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (-20 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
What is the advantage of using a PEGylated NHS biotinylation reagent?

The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule. For example, antibodies labeled with NHS-PEG4- Biotin exhibit less aggregation when stored in solution as compared to antibodies labeled with reagents having only hydrocarbon spacers. Specific labeling of cell surface proteins is another useful application for these water-soluble and membrane-impermeable reagents.

For Research Use Only. Not for use in diagnostic procedures.