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Thermo Scientific™ EZ-Link™ NHS-LC-Biotin

Catalog No. PI21336
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50 mg
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Catalog No. Quantity
PI21336 50 mg
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Catalog No. PI21336 Supplier Thermo Scientific™ Supplier No. 21336
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Description

Biotinylate protein amines (e.g., lysines) with this long-chain (22.4A), NHS-ester biotinylation reagent containing a membrane permeable spacer arm for intracellular labeling.

Thermo Scientific™ EZ-Link NHS-LC-Biotin is a long-chain, NHS-ester activated biotinylation reagent for labeling primary amines (e.g., protein lysines), whose membrane permeability enables it to be used for general intracellular labeling.

Highlights:

  • Extended spacer arm reduces steric hindrance when binding several biotinylated molecules to one avidin complex
  • Less water-soluble than Sulfo-NHS-LC-Biotin
  • Dissolve in organic solvent (DMSO or DMF) before use
  • Penetrates cell membranes because it is uncharged

Specifications

Product Type NHS-LC-Biotin
Quantity 50 mg
Content And Storage Store desiccated at 4°C. Shipped at ambient temperature.
Product Line EZ-Link
Cell Permeability Cell-Permeant
Chemical Reactivity Amine
Reactive Moiety Active Ester, Succinimidyl Ester, NHS Ester
Solubility DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
Label or Dye Biotin
Spacer Mid-length
Label Type Biotin & Analogs
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Frequently Asked Questions (FAQs)

What should I do if my EZ-Link NHS-LC-Biotin (Cat. No. 21336) is found crystalized?

The Z-Link NHS-LC-Biotin (Cat. No. 21336) should be a powder but not in crystalized form. So, in that case it is hydrolyzed and should not be used as wouldn't be reliable any more.

Can you provide the shelf-life for EZ-Link NHS-LC-Biotin?

EZ-Link NHS-LC-Biotin is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

For Research Use Only. Not for use in diagnostic procedures.