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  6. Thermo Scientific™ EZ-Link™ Maleimide-PEG2-Biotin, No-Weigh™ Format

Thermo Scientific™ EZ-Link™ Maleimide-PEG2-Biotin, No-Weigh™ Format

Catalog No. PIA39261
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PIA39261 10 x 2 mg
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Catalog No. PIA39261 Supplier Thermo Scientific™ Supplier No. A39261
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Description

Biotinylate reduced cysteines with this short, maleimide-activated, sulfhydryl-reactive biotinylation reagent containing a 2-unit PEG spacer arm to enhance water solubility.

Thermo Scientific™ EZ-Link Maleimide-PEG2-Biotin is a mid-length, maleimide-activated, sulfhydryl-reactive biotinylation reagent that contains a 2-unit ethylene glycol in its spacer arm for increased water-solubility characteristics.

  • Mid-length, maleimide-activated, sulfhydryl-reactive biotinylation reagent that contains a 2-unit ethylene glycol in its spacer arm for increased water-solubility characteristics.
  • Protein labeling - biotinylate antibodies or other proteins for use in protein methods
  • Thiol-reactive - reacts with sulfhydryls (-SH), such as the side-chain of cysteine (C)
  • Maleimide-activated - perform reactions at pH 6.5 to 7.5 in buffers such as PBS
  • Pegylated - spacer arm contains a hydrophilic, 2-unit, polyethylene glycol (PEG) group
  • Enhances solubility - pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
  • Irreversible - forms permanent thioether bonds; spacer arm cannot be cleaved
  • Solubility - can be dissolved directly in aqueous buffers for labeling reactions
  • Medium length - spacer arm (total length added to target) is 29.1 angstroms

Specifications

Product Type Maleimide-PEG2-Biotin
Quantity 10 x 2 mg
Content And Storage Store desiccated at 4°C.
Labeling Method Chemical Labeling
Shipping Condition Qualified for Ambient or Wet Ice
Product Line EZ-Link
Cell Permeability Cell-Impermeant
Chemical Reactivity Thiol
Reactive Moiety Maleimide
Solubility DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
Label or Dye Biotin
Spacer Pegylated, 29.1Å
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Frequently Asked Questions (FAQs)

How does a product with "No-Weigh" format reduce my concern over reagent stability?

The No-Weigh product format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. This format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent. Please view additional information and the video at: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/no-weigh-packaging.html

For Research Use Only. Not for use in diagnostic procedures.